The Effect of Keratin Hydrolyzate Peptide Concentration and Harvest Maturity Level on the Physiological Response and Physicochemical Quality of Siam orangess (Citrus nobilis Lour.)
DOI:
https://doi.org/10.55927/fjst.v5i9.160Keywords:
Siam Oranges, Keratin Hydrolyzate Peptide, Biostimulant, Harvest Maturity, Physicochemical Quality.Abstract
This study evaluated the effects of Keratin Hydrolyzate Peptide (KHP) concentration, harvest maturity, and their interaction on physiological and physicochemical traits of Siam oranges. A 3 × 3 factorial randomized complete block design with four blocks (36 trees) was conducted in Kintamani from October 2025 to May 2026. KHP was foliar-applied at 0, 2, and 4 mL L⁻¹. The 2 mL L⁻¹ treatment increased relative leaf water content, chlorophyll, TSS, and fruit weight. Harvest maturity affected vitamin C, TSS, TA, TSS:TA ratio, and fruit weight. P2Mp produced the highest total fruit moisture content (88.72%). Multi-season and multi-location validation is required before broader recommendation
References
Alférez, F., de Carvalho, D. U., & Boakye, D. (2021). Interplay between abscisic acid and gibberellins, as related to ethylene and sugars, in regulating maturation of non-climacteric fruit. International Journal of Molecular Sciences, 22(2), 669. https://doi.org/10.3390/ijms22020669
Colla, G., Hoagland, L., Ruzzi, M., Cardarelli, M., Bonini, P., Canaguier, R., & Rouphael, Y. (2017). Biostimulant action of protein hydrolysates: Unraveling their effects on plant physiology and the microbiome. Frontiers in Plant Science, 8, 2202. https://doi.org/10.3389/fpls.2017.02202
Dlačić, I., Chatzistathis, T., Bernobić, S., Polić Pasković, M., Ban, D., Petek, M., Popović, L., & Pasković, I. (2026). Protein hydrolyzate-based biostimulants in fruit crops: Mechanisms, agronomic effects, and practical applications. Crops, 6(2), 30. https://doi.org/10.3390/crops6020030
Eviati, Sulaeman, Herawaty, L., Anggria, L., Usman, Tantika, H. E., Prihatini, R., & Wuningrum, P. (2023). Technical Guidelines, 3rd Edition: Chemical Analysis of Soil, Plants, Water, and Fertilizers. Soil and Fertilizer Instrument Standard Testing Center. Ministry of Agriculture Repository
Gultom, L. I., Rai, I. N., & Astawa, I. N. G. (2025). Effect of reduced dosage of NPK fertilizer and application of keratin hydrolyzate peptide on yield components and yield of rice plants (Oryza sativa L.). Magna Scientia Advanced Biology and Pharmacy, 14(2), 126–132. https://doi.org/10.30574/msabp.2025.14.2.0028
Gupta, A. K., Pathak, U., Tongbram, T., Medhi, M., Terdwongworakul, A., Magwaza, L. S., Mditshwa, A., Chen, T., & Mishra, P. (2022). Emerging approaches to determine the maturity of citrus fruit. Critical Reviews in Food Science and Nutrition, 62(19), 5245–5266. https://doi.org/10.1080/10408398.2021.1883547
Hervalejo, A., Arjona-López, J. M., Ordóñez-Díaz, J. L., Romero-Rodríguez, E., Calero-Velázquez, R., Moreno-Rojas, J. M., & Arenas-Arenas, F. J. (2021). Influence of harvesting season on morphological and sensory quality, bioactive compounds, and antioxidant activity of three late-season orange- , cultivars ‘Barberina,’ ‘Valencia Midknight,’ and ‘Valencia Delta Seedless.’ Agronomy, 11(4), 673. https://doi.org/10.3390/agronomy11040673
Kaur, J., Singh, Z., Mazhar, M. S., Shah, H. M. S., & Woodward, A. (2024). Changes in physical attributes, activities of fruit-softening enzymes, cell wall polysaccharides, and fruit quality of jackfruit (Artocarpus heterophyllus Lam.) as influenced by maturation and ripening. Horticulturae, 10(12), 1264. https://doi.org/10.3390/horticulturae10121264
Lin, Y.-J., Yao, B.-T., Zhang, Q., Feng, Y.-X., & Xiang, L. (2024). Biochemical insights into proline metabolism and its contribution to the resilient cell wall structure under metal stress. Ecotoxicology and Environmental Safety, 282, 116725. https://doi.org/10.1016/j.ecoenv.2024.116725
Liu, M., Zhang, G., Wang, S., & Wang, Q. (2025). Advances in peptides for plant immunity. Plants, 14(15), 2452. https://doi.org/10.3390/plants14152452
Lu, Q., Jin, L., Wang, P., Liu, F., Huang, B., Wen, M., & Wu, S. (2023). Effects of the interaction between protein hydrolysate and arbuscular mycorrhizal fungi on citrus growth and the expression of stress-responsive genes (aquaporins and SOSs) under salt stress. Journal of Fungi, 9(10), 983. https://doi.org/10.3390/jof9100983
Lu, S., & Xiao, F. (2024). Small peptides: Orchestrators of plant growth and developmental processes. International Journal of Molecular Sciences, 25(14), 7627. https://doi.org/10.3390/ijms25147627
Magwaza, L. S., Mditshwa, A., Tesfay, S. Z., & Opara, U. L. (2017). An overview of preharvest factors affecting vitamin C content of citrus fruit. Scientia Horticulturae, 216, 12–21. https://doi.org/10.1016/j.scienta.2016.12.021
Malécange, M., Sergheraert, R., Teulat, B., Mounier, E., Lothier, J., & Sakr, S. (2023). Biostimulant properties of protein hydrolysates: Recent advances and future challenges. International Journal of Molecular Sciences, 24(11), 9714. https://doi.org/10.3390/ijms24119714
Manurung, H., Simanungkalit, F. J., & Nadapdap, M. (2024). Pengaruh tingkat kematangan dan lama penyimpanan terhadap karakteristik mutu fisikokimia jeruk Siam Madu (Citrus nobilis Lour.) pada kondisi penyimpanan dingin. Rona Teknik Pertanian, 17(1), 10–23. https://doi.org/10.17969/rtp.v17i1.37098
Nagi, L. A. L., Mayadewi, N. N. A., & Rai, I. N. (2026). The effect of peptide on the growth and yield of maize (Zea mays L.). International Journal of Multidisciplinary Research and Analysis, 9(7), 4443–4447. https://doi.org/10.47191/ijmra/v9-i7-31
Pasković, I., Popović, L., Pongrac, P., Polić Pasković, M., Kos, T., Jovanov, P., & Franić, M. (2024). Protein hydrolysates—Production, effects on plant metabolism, and use in agriculture. Horticulturae, 10(10), 1041. https://doi.org/10.3390/horticulturae10101041
Pranata, P. B. D., Wibawa, I. G. A., Raharja, M. A., & Putra, I. G. N. A. C. (2024). Pembangunan aplikasi pengenalan kualitas rasa buah jeruk Siam Kintamani menggunakan GLCM dan K-NN berbasis user centered design (UCD). JELIKU (Jurnal Elektronik Ilmu Komputer Udayana), 13(1), 167–178. https://doi.org/10.24843/JLK.2024.v13.i01.p20
Rodrigues, M., Baptistella, J. L. C., Horz, D. C., Bortolato, L. M., & Mazzafera, P. (2020). Organic plant biostimulants and fruit quality—A review. Agronomy, 10(7), 988. https://doi.org/10.3390/agronomy10070988
Rouphael, Y., Colla, G., & De Pascale, S. (2020). Protein hydrolysate-based biostimulant improves yield and fruit quality of greenhouse fresh tomatoes. Acta Horticulturae, 1271, 335–342. https://doi.org/10.17660/ActaHortic.2020.1271.46
Singh, N., Sharma, R. M., Dubey, A. K., Awasthi, O. P., Porat, R., Saha, S., Bharadwaj, C., Sevanthi, A. M., Kumar, A., Sharma, N., & Carmi, N. (2023). Assessment of harvest maturity in newly developed citrus hybrids (Citrus maxima Merr. × Citrus sinensis (L.) Osbeck) for optimal juice quality. Plants, 12(23), 3978. https://doi.org/10.3390/plants12233978
Soteriou, G. A., Rouphael, Y., Emmanouilidou, M. G., Antoniou, C., Kyratzis, A. C., & Kyriacou, M. C. (2021). Biostimulatory action of vegetable protein hydrolysate and the influence on the physicochemical characteristics of fruit in grafted watermelon. Horticulturae, 7(9), 313. https://doi.org/10.3390/horticulturae7090313
Sun, W., Shahrajabian, M. H., Kuang, Y., & Wang, N. (2024). Amino acid biostimulants and protein hydrolysates in agricultural sciences. Plants, 13(2), 210. https://doi.org/10.3390/plants13020210
Yemm, E. W., & Willis, A. J. (1954). The estimation of carbohydrates in plant extracts by anthrone. Biochemical Journal, 57(3), 508–514. https://doi.org/10.1042/bj0570508
Zhu, B., Liang, Z., Wang, D., He, C., Zhu, Z., & Yang, J. (2024). The effect of glutathione on glucosinolate biosynthesis via the sulfur assimilation pathway in pak choi under various growth conditions. Horticultural Plant Journal, 10(2), 473–487. https://doi.org/10.1016/j.hpj.2022.09.012
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